Enterolert-E

Enterolert-E

Accurate and quantifiable overnight results

               

  • Results in 24 hours.
  • Detect enterococci contamination in half the time of standard methods.
  • Eliminate tedious membrane filtration work: no confirmations, no transfer steps, no filtering required.
  • Developed for the European market and correlates with the EU Bathing Water Directive standard method for enterococci, ISO 7899-1.

Description

Overview

Easy
  • No media preparation.
  • No autoclaving.
  • No colony counting.
  • No glassware cleaning.
Rapid
  • Less than 1 minute of hands-on time.
  • Complete results in 24 hours.
Accurate
  • Correlates with ISO Method 7899-1.
  • Sensitive to 1 enterococci/100 mL.
  • Enumerates up to 2,419 enterococci per 100 mL without dilutions (with Quanti-Tray/2000).
  • Eliminates the subjective interpretation found in traditional methods.
Cost Effective
  • Up to 12-month shelf life minimizes waste.
  • 24-hour test saves incubator space.

Science

How the Enterolert-E Test works

The Enterolert-E Test uses IDEXX’s proprietary Defined Substrate Technology (DST) nutrient indicator to detect enterococci. This nutrient indicator fluoresces when metabolized by enterococci. DST improves accuracy and avoids the need for hazardous sodium azide suppressants used in traditional media.

How to use

Quantification

Step 1

Add reagent to sample.

 

Step 2

Pour into Quanti-Tray (counts from 1–200) or Quanti-Tray/2000 (counts from 1–2,419).

 

Step 3

Seal using Quanti-Tray Sealer and incubate for 24 hours at 41°C ± 0.5°C.

Step 4

Count fluorescent wells and refer to the most probable number (MPN) table.

Frequently asked questions

How can I use the Enterolert-E Test?

The Enterolert-E Test was developed for use in Europe. It correlates with the EU Bathing Water Directive standard method, ISO 7899-1.

What is the detection limit of the Enterolert-E Test?

The Enterolert-E Test detects enterococci at 1 organism/100 mL.

What is the shelf life of the Enterolert-E Test?

The Enterolert-E Test shelf life is up to 12 months from the date of manufacture.

What are the recommended storage conditions for the Enterolert-E Test?

What color and consistency should the Enterolert-E reagent powder be?

The Enterolert-E Test reagent powder should be tan in color; it should be dry and free-flowing. Any concerns about the color or integrity of the powder should be directed to IDEXX Technical Support.

Can I quantify my Enterolert-E Test results?

Yes, the Enterolert-E Test can be run with Quanti-Tray, Quanti-Tray/2000 or any multiple-tube format.

Is there a comparator for the Enterolert-E Test?

There is no comparator for the Enterolert-E Test. For comparison, use a negative control when interpreting results.

What are the disposal requirements for the Enterolert-E Test sample?

Check with your local, state, and/or federal authorities for proper disposal of bacteriological biohazard materials at your facility.

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