Colisure*

Detect coliforms/E. coli with the convenience of a long-read window

Simultaneously detects both total coliforms and Escherichia coli; positive results have a distinct magenta color. Results valid through 48 hours.

Distinct magenta color means there’s no mistaking a positive result.
The Colisure Test also suppresses high levels of noncoliforms, including Aeromonas.
The Colisure Test is U.S. EPA approved and included in Standard Methods for Examination of Water and Wastewater.

Overview

Easy

Distinct magenta color is easy to see.
Ease of use simplifies training.
Unit-dosed packaging eliminates media preparation.
Quality Control (QC) procedure can be done in 15 minutes.

Rapid

Less than 1 minute of hands-on time.
Detects coliforms and E. coli simultaneously in 24 hours or less.
No confirmations needed.
No glassware cleaning or colony counting.

Accurate

Definitive results at 24 hours; can be read up to 48 hours.
Identifies E. coli specifically, eliminating unneeded public notification due to non-target organisms.
Suppresses up to 2 million heterotrophs per 100 mL.
Eliminates the subjective interpretation found in traditional methods.
Detects a single viable coliform or E. coli per sample.

Economical

Minimizes evening and weekend work.
Up to 12-month shelf life at room temperature.

Flexible

The Colisure Test can be used for presence/absence (P/A) or quantification testing.
Quanti-Tray provides counts to 200/100 mL without dilutions.
Quanti-Tray/2000 provides counts to 2,419/100 mL without dilutions.

Science

How the Colisure Test works

The Colisure Test uses proprietary Defined Substrate Technology (DST) nutrient indicators CPRG and MUG to detect total coliforms and E. coli. Coliforms use the β-galactosidase enzyme to metabolize CPRG and change it from yellow to magenta. E. coli use β-glucuronidase to metabolize MUG and create fluorescence.

How to use

Presence/Absence

Step 1

Add reagent to sample and incubate at 35 ± 0.5°C for 24 hours.

Step 2

Read results:

Yellow = negative
Magenta = total coliforms
Magenta/fluorescent = E. coli

Quantification

Step 1

Add reagent to sample.

Step 2

Pour into Quanti-Tray (counts from 1–200) or Quanti-Tray/2000 (counts from 1–2,419).

Step 3

Seal in Quanti-Tray Sealer and place in incubator at 35 ± 0.5°C for 24 hours.

Step 4: Quanti-Tray

Quanti-Tray—Read results:

Magenta wells = total coliforms
Magenta/fluorescent wells = E. coli
Count positive wells and refer to MPN table

Step 4: Quanti-Tray/2000

Quanti-Tray/2000—Read results:

Magenta wells = total coliforms
Magenta/fluorescent wells = E. coli
Count positive wells and refer to MPN table

Colisure*

Detect coliforms/E. coli with the convenience of a long-read window